Detailed Protocols
(Routinely Used by Razani Lab)

Cell Isolation and Differentiation Protocols

Peritoneal Macrophage Isolation

Human Monocyte-derived Macrophage Isolation

Bone Marrow-derived Macrophage Isolation

Adipocyte Differentiation of MEFs (Coming Soon)

Bone Marrow Transplantation in Mice

Adipocyte Differentiation of Stromal Vascular Fraction (Coming Soon)

Serum Triglyceride, Cholesterol, Glucose, and Free Fatty Acid Assay

Tissue Homogenization

(Using Beads) (Coming Soon)

Insulin Tolerance Test (ITT) Glucose Tolerance Test (GTT)

Isolation of Total RNA (Using Columns) (Coming Soon)

Metabolic Chamber Analysis (Phenomaster)

Lentiviral Transduction